Selective Biochip with portable analyzer for assessment of insulin resistance and the metabolic syndrome (BIOSIM).

Programme PN III: Programme 2- Increasing the Romanian Economy Competitiveness by Research, Development and Innovation.

Project Type: Transfer from economic operator.
Project financed by the state budged and co-financed by DDS Diagnostic.
Contract No. 50PTE/2016
Period: 2016-2018
Coordinator: S.C. DDS DIAGNOSTIC S.R.L Bucharest.
Project Director: Phd. Dana Stan

The main purpose of this project is to develop and certify a new technology for the manufacturing of portable selective biochips and of the portable tester, for the assessment of the insulin resistance and a metabolic syndrome, up to TRL5.

Main Targets of the Project:

1. Development of a prototype based on new technologies for the manufacturing of integrated biochips, starting from the outcomes of the scientific research carried out by DDS Diagnostic and IMT.
2. Development of a partnership between the coordinating institution (IMM) and the research institutions for the creation of state of the art technology, for the quick transfer thereof to the industrial partner to be traded on the internal and external market.
3. Development of a new, accessible, easy to use technology with quick responses, low costs and immediate application in the early assessment and diagnosis of the insulin resistance and metabolic syndrome.
4. Testing and classification of the new technology for the detection of insulin resistance and metabolic syndrome assessment biomarkers.
5. Interpretation, validation of outcomes and correlation thereof with current clinical analysis; Transfer of technology.
6. Outcome Dissemination.

Coordinator: S.C. DDS Diagnostic S.R.L
Project Director: Dana Stan
Partner: National Institute for Research and Development in Microtechnologies, Bucharest (IMT)
Project Manager: Dr. Carmen Aura Moldovan

Development of a prototype including selective biochips with synthetic recognition elements and of a portable system for the simultaneous quantity detection of several biomarkers for the early diagnosis of the insulin resistance and metabolic syndrome.

Stage 1 – 2016 - Design, biochemical reaction protocols, optimisation. During Stage 1 of the BIOSIM Project, the partners, IMT and DDS, executed the design and simulation of the microfluidic chip for multiple sensors with microchannels allowing the application of the sample to the reaction and detection area.

- Reaction protocol on the electrode areas.
- Optimisation and mechanical assembly of the biochip components for manufacturing.
- Construction of the Project website.

Deliverable: Technical Report on the Reaction protocol and Microfluidic Chip Design Report.

Stage 2 – 2017 - Manufacturing, testing and optimisation of prototype components (biochips and electronic modules).

During Stage 2 of the project, the partners, IMT and DDS, executed:
- Manufacturing and testing of micro-transducers and electronic module;
- Achievement, testing and optimisation of conductive/non-conductive polymer films using the MIP technique on gold microelectrodes;
- Innovation Report;
- Dissemination.

Etapa 3 – 2018 - Testare prototip si Validare tehnologie.

Articles:

1.Carmen-Marinela MIHĂILESCU, Mihaela SAVIN, Dana STAN, Carmen MOLDOVAN, George MUSCALU, Silviu DINULESCU, „Selective Biochip for the Assessment of the Insulin Resistance and Metabolic Syndrome (SM)” Revista Română de Medicină de Laborator (Romanian Laboratory Medicine Magazine) . Supplement to Vol. 25, No. 2, April 2017;
2.Mihailescu Carmen –Marinela, Stan Dana, Carmen Aura Moldovan, Silviu Dinulescu, Patrascu Florentina „“Label free” immunosensor for metabolic syndrome investigation” Analele Universitatii Titu Maiorescu, sectiunea Medicina, ISSN: 1584-4781, accepted for publication.

Conferences:

1. Carmen-Marinela MIHĂILESCU, Mihaela SAVIN, Dana STAN, Carmen MOLDOVAN, George MUSCALU, Silviu DINULESCU, „ Selective Biochip for the Assessment of the Insulin Resistance and Metabolic Syndrome (SM)”, 2nd Conference of the Romanian Association of Laboratory Medicine, P42 [may 2017]; 2.Mihailescu Carmen –Marinela, Stan Dana, Carmen Aura Moldovan, Silviu Dinulescu, Patrascu Florentina „“Label free” immunosensor for metabolic syndrome investigation”, 11th edition International Conference Education and Creativity for a knowledge based society, oral presentation, November 16 - 18, 2017.

Patent Applications:

1.C.M. Mihailescu, D. Stan, „Procedeu de obtinere sablon selectiv pentru detectia electrochimica “label free” a adiponectinei din probe biologice umane” (Procedure for the obtaining of a selective pattern for the “label free” electrochemical detection of the adiponectin on biological human samples”, submitted on 27.11.2017, under no. A00983/27.11.2017.

Scientific seminar:

1. Theme: „Electrochemical Detection Biosensor for the Identification of Metabolic Syndrome related Proteins”.
Organisers: SC DDS Diagnostic SRL and IMT-Bucharest
Date: 17.11.2017
Participants: 15 participants, specialists in clinical medical laboratories and medical product distribution companies.