Determination of reactive C protein (PCR) by agglutination method (LATEX)  

The latex CRP test is a rapid agglutination test on the blade for the qualitative and semi-quantitative detection of reactive C protein in human serum samples. PCR is an acute phase reactant that increases rapidly but nonspecifically in case of inflammation, tissue damage or infections.

This detection method uses a suspension of latex particles coated with anti-human C-reactive human antibody (antibody) specific antibodies to be contacted with the patient’s serum (containing PCR). If the agglutination reaction occurs, the test is considered positive.

 

INSTRUCTIONS FOR THE QUALITATIVE METHOD

 

MATERIAL PREPARATION

Place all the necessary materials at the room temperature on the work table. Read the working instructions.

The number of the patient is noted on the card. Use a 40μl pipette.

Samples to be tested should not be contaminated, haemolized or lipemic.

 

SAMPLE WORK

Easily shake the positive control

Place a positive control drop in the first circle

Place a negative control drop in the second circle

Place 40μl of patient samples in the rest of the circles

Gently stir the latex suspension

Place a drop of the latex suspension in each circle next to the test sample

Mix the test sample with the latex solution using the beater and spread over the entire surface of the circle. Use a new mixer at each sample

Mix the mixture by rotating the card with about 100 rpm for about 2 minutes and note the moment when the sample agglutinates

If reading is done after 2 minutes, false positive results may occur

 

Interpretation

The presence or absence of agglutination is examined macroscopically. The presence of visible agglutination indicates a concentration greater than or equal to 6 mg / l (positive result) while the lack of agglutination is less than 6 mg / l.

 

Semi-quantitative method

This method uses serial dilutions with patient serum and saline solution. After the dilutions are made, place a drop of latex and mix.

 

Sources of errors

Bacterial contamination of controls and samples

Refrigeration / melting of reagents

The traces of detergent on the reaction plates after washing

 

RESULT