National Congress of the Romanian Laboratory of Medical Medicine with international participation (AMLR)

The conference is organized under the auspices of the International Federation of Clinical Chemistry IFCC and the European Federation of Laboratory Medicine EFLM, in collaboration with UMF Bucharest, UMF Târgu Mureş, UMF Iuliu Haţieganu Cluj Napoca, UMF Timişoara and UMF Iaşi, having as professional partner companies the Romanian Society of Microbiology and the Romanian Society of Hematology.

At the congress,  DDS Diagnostic team will hold two oral presentations

FUNCTIONALIZED INTERDIGITATED SURFACES FOR ELECTROCHEMICAL DETECTION OF  CD4+ LYMPHOCYTES COUNT

Dr. Chim. Dana Stan talks about the detection of CD4 + and CD8 + lymphocytes in the blood (useful in diagnosing HIV infection) with a miniaturized device containing a series of interdigitated electrodes of predetermined size (depending on lymphocyte size). The electrodes were functionalized with antibodies specific for the detection of lymphocyte subpopulations, thus aiming at obtaining a capture chamber for each subpopulation. The purpose of these tests was to demonstrate the functionality of the electrodes used for electrochemical detection of CD4 + lymphocytes.

This work was supported by a grant of the Romanian National Authority for Scientific Research and Innovation, CNCS/CCCDI –UEFISCDI, project number PN-III-P2-2.1-PTE-2016-0145, within PNCDI III and cofinanced by DDS Diagnostic SRL.

Key words: T lymphocytes, CD4 +, interdigitated electrodes

 

“ARTIFICIAL ANTIBODIES” USED FOR THE SELECTIVE DETECTION OF HUMAN SERUM ADIPONECTINE

In this presentation Dr. Chim. Carmen Mihailescu will talk about molecular printing technology (MIP) with the development of biomimetic sensors with applications in laboratory diagnostics “in vitro”. The targeted application is the determination of human serum adiponectin, a protein that regulates fat levels and controls obesity.

The method consists in electropolymerizing the phenol on the gold surface of an electrochemical sensor in the presence of adiponectin followed by the extraction of adiponectin from the resulting polymeric matrix, a process that generates complementary cavities as a form and size with adiponectin so-called “artificial antibodies”. These antibodies capture adiponectin from the human serum generating a measurable electrochemical signal that is directly proportional to the concentration.

This work was supported by a grant of the Romanian National Authority for Scientific Research and Innovation, CNCS/CCCDI –UEFISCDI, project number PN-III-P2-2.1-PTE-2016-0165, within PNCDI III and cofinanced by DDS Diagnostic SRL.

 Key words: adiponectin, artificial antibodies, molecular printing technology

 

We also look forward to meet you at our stand at the equipment, reagents and laboratory supplies exhibition where you can find out more about our products and services.

For more information about the congress please visit  www.raml-conference.ro